Dynamic accumulation of biomass and secondary metabolites of Pesudostellaria heterophylla in Guizhou province / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 1346-1351, 2013.
Article
in Zh
| WPRIM
| ID: wpr-860266
Responsible library:
WPRO
ABSTRACT
OBJECTIVE: To study the dynamic accumulation of biomass and secondary metabolites from different cultivation forms, reproduced by different modes in different growth periods of Pesudostellaria heterophylla in Shibing district, Guizhou province, in order to provide guidance for variety breeding and rational harvest method of P. heterophyila. METHODS: Taking three cultivation forms and two kinds of breeding materials as the main research subjects, the dynamic accumulation of biomass was periodically recorded with growth period change, and the contents of pseudostellarin B in the roots of P. heterophyila were analyzed by HPLC. RESULTS: The underground biomass in cultivation form of vegetative propagation XZ-1, XZ-2 and XZ-3 reached the highest levels of 9.86, 3.61 and 10.40 g · plant- in early July, late June and the beginning of July, respectively. The content of pseudostellarin B reached the criterion of Chinese Pharmacopoeia only in XZ-1 in early July, which was 0.0213%. In the harvest time, the contents of biomass in XZ-1 and XZ-3 were significantly higher than that in XZ-2, while the contents of pseudostellarin B in XZ-1 and XZ-2 were significantly higher than that in XZ-3. The content of biomass in sexual reproduction material, XZ-1Y, was significantly lower than that in the vegetative propagation one, XZ-1 of the same cultivation form, but the content of pseudostellarin B in XZ-IY was significantly higher than that in XZ-1. CONCLUSION: According to the comprehensive consideration of the contents of biomass and secondary metabolites, the XZ-1 of P. heterophyila has better quality, and it is worth popularizing and being used for variety breeding. The optimal harvest time of Pesudostellaria heterophyila is about 10 days after the plant is withered.
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WPRIM
Language:
Zh
Journal:
Chinese Pharmaceutical Journal
Year:
2013
Type:
Article