Comparison of triptolide contents in different parts of Tripterygii radix / 国际中医中药杂志

ABSTRACT

Objective:To determine the content of triptolide in different parts of Tripterygii radix by high performance liquid chromatography. Methods:Tripterygii radix was determined by high performance liquid chromatography with Agilent Technologies C18 column (4.6 mm × 250 mm, 5 μm), acetonitrile-water (33:67) as mobile phase, flow rate of 1 ml/min, the column temperature of 30 ℃, injection volume of 20 ml and wavelength of 218 nm. Results:The linear relationship of triptolide was good in the range of 0.204-2.040 μg ( r=0.999 9), and the average recovery rate was 93.35%; RSD was 1.56% ( n=6). The lignin content in different parts of root was higher than that of the skin. Conclusions:The method is simple, rapid and accurate, which could be used to determine the content of triptolide in Tripterygii radix.