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Melittin-K1 reverses multidrug resistance of human hepatocellular carcinoma BEL-7402/5-FU cells / 中华普通外科杂志
Chinese Journal of General Surgery ; (12): 882-886, 2020.
Article in Chinese | WPRIM | ID: wpr-870539
ABSTRACT

Objective:

To investigate the mechanism by which Melittin-K1 reverses multidrug resistance of BEL-7402/5-FU cells.

Methods:

CCK-8 assay was used to evaluate the effect of Melittin-K1 on the growth of BEL-7402/5-FU cells and to explore whether Melittin-K1 could reverse the drug resistance of BEL-7402/5-FU cells. The expression of MDR1 mRNA level was detected by real-time fluorescence quantitative PCR assay. The flow cytometry was used to measure the expression of P-glycoprotein (P-gp) on the cell membrane surface and the accumulation of rhodamine-123 in cells.

Results:

Melittin-K1 significantly inhibited the growth of BEL-7402/5-FU cells in vitro in a time and dose-dependent manner. Melittin-K1 suppressed the level of MDR1 mRNA and inhibited the surface expression and function of P-gp in BEL-7402/5-FU cells.

Conclusions:

Melittin-K1, a novel peptide, exhibited the activity of reversing multidrug resistance of liver cancer cells.
Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of General Surgery Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of General Surgery Year: 2020 Type: Article