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Determination of 11 main components in Hanshi Yufei decoction, a COVID-19 empirical prescription by UHPLC-MS/MS / 药学实践杂志
Journal of Pharmaceutical Practice ; (6): 148-151, 2021.
Article in Chinese | WPRIM | ID: wpr-875676
ABSTRACT
Objective To establish a method to determine 11 main components in Hanshi yufei decoction. Methods The method adopted UHPLC-MS/MS with an Agilent ZORBAX SB-C18 (3.5 μm,2.1 mm×150 mm) column. The mobile phase was consisted of 0.2% formic acid plus 10 mmol/L ammonium acetate aqueous solution(A) - acetonitrile(B) and gradient elution (0–0.6 min, 80%–40%A; 0.6–1 min, 40%–30%A; 1–4.3 min, 30%–5%A) at 0.3 ml/min. The column temperature was 40 ℃ and 11 main components including vanillic acid, magnolol, honokiol, wogonin, sophorin, 6-gingerol, citrinin, qianghuo alcohol, nobiletin, nodakenin, and hesperidin were quantified in a multiple reaction monitoring mode. The reserpine was the standard. Results The 11 main components in Hanshi Yufei decoction had a good linear relationship within their concentration range (r>0.98), and the average recovery was 93.11%~111.73%. Conclusion The UHPLC-MS/MS method established in this experiment is easy to operate and has good reproducibility, which provides a laboratory basis for the quality control of Hanshi Yufei decoction.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Pharmaceutical Practice Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Pharmaceutical Practice Year: 2021 Type: Article