Strategies and tools for metabolic engineering in Bacillus subtilis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1619-1636, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-878658
ABSTRACT
As a typical food safety industrial model strain, Bacillus subtilis has been widely used in the field of metabolic engineering due to its non-pathogenicity, strong ability of extracellular protein secretion and no obvious codon preference. In recent years, with the rapid development of molecular biology and genetic engineering technology, a variety of research strategies and tools have been used to construct B. subtilis chassis cells for efficient synthesis of biological products. This review introduces the research progress of B. subtilis from the aspects of promoter engineering, gene editing, genetic circuit, cofactor engineering and pathway enzyme assembly. Then, we also summarized the application of B. subtilis in the production of biological products. Finally, the future research directions of B. subtilis are prospected.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bacillus subtilis
/
Bacterial Proteins
/
Promoter Regions, Genetic
/
Metabolic Engineering
/
Gene Editing
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article
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