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Master genes and co-expression network analysis in peripheral blood mononuclear cells of patients with gram-positive and gram-negative sepsis / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences ; (6): 732-742, 2020.
Article in Chinese | WPRIM | ID: wpr-879935
ABSTRACT
OBJECTIVE@#To investigate the functional pathways enriched and differentially expressed genes (DEGs) in peripheral blood mononuclear cells (PBMCs) of patients with gram-positive and gram-negative sepsis.@*METHODS@#Dataset GSE9960 obtained from NCBI GEO database containing PBMC samples from 16 non-infectious systematic inflammatory response syndrome (SIRS) patients, 17 gram-positive septic patients and 18 gram-negative septic patients were included in the study. Functional pathway annotations were conducted by gene set enrichment analysis and weighted gene co-expression network analysis. DEGs were filtered and master DEGs were then validated in PBMCs of gram-positive septic, gram-negative septic and non-infectious SIRS patients.@*RESULTS@#The enriched gene sets in gram-positive sepsis and gram-negative sepsis were significantly different. The results indicated the opposite co-expression networks in SIRS and gram-negative sepsis, and the entirely different co-expression networks in gram-positive and gram-negative sepsis. Furthermore, we validated that @*CONCLUSIONS@#The results indicate that there are differences in the mechanism and pathogenesis of gram-positive and gram-negative sepsis, which may provide potential markers for sepsis diagnosis and empirical antimicrobial therapy.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Leukocytes, Mononuclear / Biomarkers / Gram-Positive Bacterial Infections / Gram-Negative Bacterial Infections / Sepsis / Gene Expression Profiling Limits: Humans Language: Chinese Journal: Journal of Zhejiang University. Medical sciences Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Leukocytes, Mononuclear / Biomarkers / Gram-Positive Bacterial Infections / Gram-Negative Bacterial Infections / Sepsis / Gene Expression Profiling Limits: Humans Language: Chinese Journal: Journal of Zhejiang University. Medical sciences Year: 2020 Type: Article