Effect of IL-15 addition on asbestos-induced suppression of human cytotoxic T lymphocyte induction
Environmental Health and Preventive Medicine
;
: 50-50, 2021.
Article
in English
| WPRIM
| ID: wpr-880368
ABSTRACT
BACKGROUND@#Asbestos fibers possess tumorigenicity and are thought to cause mesothelioma. We have previously reported that exposure to asbestos fibers causes a reduction in antitumor immunity. Asbestos exposure in the mixed lymphocyte reaction (MLR) showed suppressed induction of cytotoxic T lymphocytes (CTLs), accompanied by a decrease in proliferation of CD8@*METHODS@#For MLR, human peripheral blood mononuclear cells (PBMCs) were cultured with irradiated allogenic PBMCs upon exposure to chrysotile B asbestos at 5 μg/ml for 7 days. After 2 days of culture, IL-15 was added at 1 ng/ml. After 7 days of MLR, PBMCs were collected and analyzed for phenotypic and functional markers of CD8@*RESULTS@#IL-15 addition partially reversed the decrease in CD3@*CONCLUSION@#These findings indicate that CTLs induced upon exposure to asbestos possess dysfunctional machinery that can be partly compensated by IL-15 supplementation, and that IL-15 is more effective in the recovery of proliferation and granzyme B levels from asbestos-induced suppression of CTL induction compared with IL-2.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asbestos
/
Lymphocyte Activation
/
T-Lymphocytes, Cytotoxic
/
CD8-Positive T-Lymphocytes
/
Interleukin-15
Limits:
Humans
Language:
English
Journal:
Environmental Health and Preventive Medicine
Year:
2021
Type:
Article
Similar
MEDLINE
...
LILACS
LIS