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Effects of Oridonin-loaded Chitosan Graphene Oxide on the Proliferation and Apoptosis of A 549 Cells / 中国药房
China Pharmacy ; (12): 1589-1595, 2021.
Article in Chinese | WPRIM | ID: wpr-881460
ABSTRACT
OBJECTIVE:To study the effects of chitosan graphene oxide car rier(CS-GO)loaded with oridonin (CS-GO- oridonin)on the proliferation and apoptosis of human lung cancer A 549 cells. METHODS :Taking A 549 cells as objects ,the survival rate of cells were detected by CCK- 8 method after treated with different concentrations of CS-GO (3,6,12,24,48 μg/mL)and CS-GO-oridonin loaded with same mass of oridonin (3,6,12,24,48 μg/mL,by the weight of oridonin ,the same below). IC 50 of CS-GO-oridonin was calculated. The cell morphology were observed by microscope after treated with CS-GO and CS-GO-oridonin(both 32 μg/mL)for 2,4,10,24 h. The uptake of CS-GO ,oridonin,CS-GO-orionin(all 32 μg/mL)by cells was observed with fluorescence labeling method. The apoptosis of cells and the content of ROS were observed by flow cytometry after treated with different concentrations of CS-GO (16,32,64 μg/mL)and CS-GO-oridonin (16,32,64 μg/mL). The expression of anti-apoptosis related proteins (Mcl-1,Bax and Bak )were detected by Western blot. RESULTS :After treated with different concentrations of CS-GO ,the survival rate of cells was still above 90% ;after treated with different concentrations of CS-GO-oridonin,the survival rate of cells showed a downward trend ,and was significantly lower than that of CS-GO group (P< 0.01);IC50 of CS-GO-oridonin was 32.61 μg/mL. After CS-GO treatment,the cell morphology did not change significantly ;after CS-GO-oridonin treatment ,the cells shrunk and fell off in clusters ,and the suspended matter increased ;the fluorescence of oridonin and CS-GO-orionin taken up by cells was enhanced than CS-GO. Compared with blank group ,there was no significant change in the apoptosis rate ,the content of ROS and the expression of apoptosis-related protein in 16,32,64 μg/mL CS-GO groups(P>0.05);apoptosis rate ,the content of ROS ,the protein expression of Bax and Bak in 16,32,64 μg/mL CS- GO-oridonin groups were increased significantly ,while the protein expression of Mcl- 1 were decreased significantly. Above indexes were significantly high er or lower than the same concentration CS-GO group (P<0.05). CONCL USIONS:CS-GO dose not affect the proliferation and apoptosis of A 549 cells;CS-GO-oridonin has obvious inhibition and apoptosis promoting effect on cells ,which may be related to increasing ROS production and regulating the expression of apoptosis related proteins.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2021 Type: Article