Study on the stability of SARS-CoV-2 at different temperatures / 上海预防医学
Shanghai Journal of Preventive Medicine
;
(12): 818-823, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-887144
ABSTRACT
Objective:
To observe the stability of severe acute respiratory syrdrome coronavirus (SARS-CoV-2) in cell cultures at different temperatures so as to provide basic data and scientific basis for the research and control of COVID-19 epidemic.Methods:
The Vero E6 cells inoculated with SARS-CoV-2. According to TCID50, SARS-CoV-2 with different dilution (10-1, 10-3, 10-5, 10-6)were stored at 37 °C, 22.5 °C, and 4 °C for one to seven days, and then infectious titer was determined by micro cytopathogenic effect assay, observing cytopathic effect (CPE), and real-time fluorescence quantitative testing.Results:
SARS-CoV-2 was stable under 4 °C. The infectivity of high concentration (10-1 dilution) under 22.5 °C for seven days gradually decreased, while lower concentration completely lost infectivity after one day. The virus lost infectivity when stored at 37 °C for more than one day.Conclusion:
SARS-CoV-2 is highly stable at 4 °C, sensitive to heat, and related to virus concentration.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Shanghai Journal of Preventive Medicine
Year:
2021
Type:
Article
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