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Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma / 生物工程学报
Chinese Journal of Biotechnology ; (12): 2293-2306, 2021.
Article in Chinese | WPRIM | ID: wpr-887797
ABSTRACT
Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: B-Lymphocytes / Hybridomas / Antibodies, Monoclonal / Antigens Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: B-Lymphocytes / Hybridomas / Antibodies, Monoclonal / Antigens Limits: Animals Language: Chinese Journal: Chinese Journal of Biotechnology Year: 2021 Type: Article