Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2293-2306, 2021.
Article
in Chinese
| WPRIM
| ID: wpr-887797
ABSTRACT
Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
B-Lymphocytes
/
Hybridomas
/
Antibodies, Monoclonal
/
Antigens
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2021
Type:
Article
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