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Quantitation and content investigation of bilirubin in cultured cow-bezoar / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2852-2856, 2021.
Article in Chinese | WPRIM | ID: wpr-887959
ABSTRACT
A determination method for bilirubin in cultured cow-bezoar was developed in this study, with which the bilirubin in 15 batches of samples was quantified. The samples were first processed with 10% oxalic acid solution for the conversion of bilirubin from conjugated to unconjugated, followed by the extraction with dichloromethane. Then the obtained sample solutions were analyzed at 450 nm by HPLC[chromatographic column Agilent TC-C_(18)(4.6 mm × 250 mm, 5 μm); mobile phase acetonitrile and 1% glacial acetic acid aqueous solution(95∶5); flow rate 1.0 mL·min~(-1)]. The bilirubin content in the 15 batches of cultured cow-bezoar was ranged from 21.9% to 41.7% with the average of 32.4%. The proposed method is accurate and reliable, thus making it suitable for the quantitation of bilirubin in cultured cow-bezoar and its quality assessment and control.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bezoars / Bilirubin / Drugs, Chinese Herbal / Chromatography, High Pressure Liquid Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2021 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bezoars / Bilirubin / Drugs, Chinese Herbal / Chromatography, High Pressure Liquid Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2021 Type: Article