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Role of Ski in Inflammatory Factor Release in Activated Astrocytes / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice ; (12): 802-806, 2018.
Article in Chinese | WPRIM | ID: wpr-923645
ABSTRACT
@#Objective To investigate the effect of Ski on the secretion of inflammatory cytokines from activated astrocytes. Methods Astrocytes were obtained from cerebral cortex of a three-day old Sprague-Dawley rat and cultured in vitro. They were divided into blank group, control group and siRNA group. The Ski gene was silenced in siRNA group. The expression of Ski was tested with Western blotting and immunofluorescence 48 hours later. Then the astrocytes were stimulated with lipopolysaccharide for 24 hours. The secretion of tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) in activated astrocytes was detected with ELISA. Results The expression of Ski protein reduced in the siRNA group (P<0.001), as well as the secretion of TNF-α and IL-1β (P<0.001). Conclusion Ski may play a role in inflammatory response of astrocyte.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Rehabilitation Theory and Practice Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Rehabilitation Theory and Practice Year: 2018 Type: Article