Caprylic Acid Improves Lipid Metabolism, Suppresses the Inflammatory Response and Activates the ABCA1/p-JAK2/p-STAT3 Signaling Pathway in C57BL/6J Mice and RAW264.7 Cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 95-106, 2022.
Article
in English
| WPRIM
| ID: wpr-927639
ABSTRACT
OBJECTIVE@#This study aimed to investigate the effects of caprylic acid (C80) on lipid metabolism and inflammation, and examine the mechanisms underlying these effects in mice and cells.@*METHODS@#Fifty-six 6-week-old male C57BL/6J mice were randomly allocated to four groups fed a high-fat diet (HFD) without or with 2% C80, palmitic acid (C160) or eicosapentaenoic acid (EPA). RAW246.7 cells were randomly divided into five groups normal, lipopolysaccharide (LPS), LPS+C80, LPS+EPA and LPS+cAMP. The serum lipid profiles, inflammatory biomolecules, and ABCA1 and JAK2/STAT3 mRNA and protein expression were measured.@*RESULTS@#C80 decreased TC and LDL-C, and increased the HDL-C/LDL-C ratio after injection of LPS. Without LPS, it decreased TC in mice ( P < 0.05). Moreover, C80 decreased the inflammatory response after LPS treatment in both mice and cells ( P < 0.05). Mechanistic investigations in C57BL/6J mouse aortas after injection of LPS indicated that C80 resulted in higher ABCA1 and JAK2/STAT3 expression than that with HFD, C160 and EPA, and resulted in lower TNF-α, NF-κB mRNA expression than that with HFD ( P < 0.05). In RAW 264.7 cells, C80 resulted in lower expression of pNF-κBP65 than that in the LPS group, and higher protein expression of ABCA1, p-JAK2 and p-STAT3 than that in the LPS and LPS+cAMP groups ( P < 0.05).@*CONCLUSION@#Our studies demonstrated that C80 may play an important role in lipid metabolism and the inflammatory response, and the mechanism may be associated with ABCA1 and the p-JAK2/p-STAT3 signaling pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Caprylates
/
Signal Transduction
/
Cholesterol
/
STAT3 Transcription Factor
/
Lipid Metabolism
/
Janus Kinase 2
/
Diet, High-Fat
/
ATP Binding Cassette Transporter 1
/
Inflammation
/
Macrophages
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2022
Type:
Article
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