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Comparison of chemical components between aerial and underground parts of Coptis chinensis based on UPLC-Q-TOF-MS~E technology / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 980-987, 2022.
Article in Chinese | WPRIM | ID: wpr-928017
ABSTRACT
The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS~E) technology was employed to compare the chemical components between the aerial and underground parts of Coptis chinensis samples from different batches. According to the retention time, molecular ion peak, and LC-MS~E fragment information of the reference substances and available literature, we identified a total of 40 components. Thirty-three and 31 compounds were respectively identified in the underground part(taproots) and the aerial part(stems and leaves) of C. chinensis. Among them, 24 compounds, including alkaloids(e.g., berberine and jatrorrhizine) and phenolic acids(e.g., chlorogenic acid, quinic acid, and tanshinol), were common in the two parts. In addition, differential components were also identified, such as magnoline glucoside in the underground part and(±) lariciresionol-4-β-D-glucopyranoside in the aerial part. The analysis of fragmentation pathways based on spectra of reference substances indicated the differences among samples of different batches. Furthermore, we performed the principal component analysis(PCA) for the peak areas of C. chinensis in different batches. The results showed that the underground part and the aerial part were clearly clustered into two groups, indicating that the chemical components contained in the two parts were different. Furthermore, the results of partial least squares discriminant analysis(PLS-DA) identified 31 differential compounds(VIP value>1) between the underground part and the aerial part, mainly including alkaloids, phenolic acids, lignans, and flavonoids. This study proves that C. chinensis possesses great development potential with multiple available compounds in stems and leaves. Moreover, it sheds light on for the development and utilization of non-medicinal organs of C. chinensis and other Chinese medicinal herbs.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Technology / Chromatography, High Pressure Liquid / Spectrometry, Mass, Electrospray Ionization / Tandem Mass Spectrometry / Coptis chinensis Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2022 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Technology / Chromatography, High Pressure Liquid / Spectrometry, Mass, Electrospray Ionization / Tandem Mass Spectrometry / Coptis chinensis Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2022 Type: Article