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Ershiwuwei Shanhu Pills regulate Akt/mTOR/GSK-3β signaling pathway to alleviate Alzheimer's disease mice / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2074-2081, 2022.
Article in Chinese | WPRIM | ID: wpr-928147
ABSTRACT
The present study investigated the mechanism of the Tibetan patent medicine Ershiwuwei Shanhu Pills(ESP) in alleviating Alzheimer's disease in mice via Akt/mTOR/GSK-3β signaling pathway. BALB/c mice were randomly assigned into a blank control group, a model group, low(200 mg·kg~(-1)), medium(400 mg·kg~(-1)) and high(800 mg·kg~(-1)) dose groups of ESP, and donepezil hydrochloride group. Except the blank control group, the other groups were given 20 mg·kg~(-1) aluminum chloride by gavage and 120 mg·kg~(-1) D-galactose by intraperitoneal injection for 56 days to establish Alzheimer's disease model. Morris water maze was used to detect the learning and memory ability of mice. The level of p-tau protein in mouse hippocampus and the levels of superoxide dismutase(SOD), malondialdehyde(MDA), catalase(CAT), and total antioxidant capacity(T-AOC) in hippocampus and serum were detected. Hematoxylin-eosin staining and Nissl staining were performed for the pathological observation of whole brain in mice. TdT-mediated dUTP nick-end labeling(TUNEL) staining was employed for the observation of apoptosis in mouse cortex. Western blot was adopted to detect the protein levels of p-mTOR, p-Akt, and GSK-3β in the hippocampus. Compared with the model group, the ESP groups showcased alleviated pathological damage of the whole brain, decreased TUNEL positive cells, reduced level of p-tau protein in hippocampus, and risen SOD, CAT, and T-AOC levels and declined MDA level in hippocampus and serum. Furthermore, the ESP groups had up-regulated protein levels of p-mTOR and p-Akt while down-regulated protein level of GSK-3β in hippocampus. Therefore, ESP can alleviate the learning and memory decline and oxidative damage in mice with Alzheimer's disease induced by D-galactose combined with aluminum chloride, which may be related to Akt/mTOR/GSK-3β signaling pathway.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Superoxide Dismutase / Plant Extracts / Signal Transduction / Tau Proteins / Proto-Oncogene Proteins c-akt / TOR Serine-Threonine Kinases / Alzheimer Disease / Glycogen Synthase Kinase 3 beta / Aluminum Chloride / Galactose Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2022 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Superoxide Dismutase / Plant Extracts / Signal Transduction / Tau Proteins / Proto-Oncogene Proteins c-akt / TOR Serine-Threonine Kinases / Alzheimer Disease / Glycogen Synthase Kinase 3 beta / Aluminum Chloride / Galactose Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2022 Type: Article