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Identification and analysis of 2 Corynebacterium diphtheria strains in Guangdong Province / 中华预防医学杂志
Chinese Journal of Preventive Medicine ; (12): 427-432, 2022.
Article in Chinese | WPRIM | ID: wpr-935303
ABSTRACT

Objective:

To identify and analyze two strains of C. diphtheriae in Guangdong Province by combining whole genome sequencing with traditional detection methods.

Methods:

The C. diphtheriae was isolated from Guangzhou in 2010 and Zhuhai in 2020 respectively. Isolates were identified by API Coryne strips and MALDI-TOF-MS. Genomic DNA was sequenced by using Illumina. The assembly was performed for each strain using CLC software. J Species WS online tool was used for average nucleoside homology identification, then narKGHIJ and tox gene were detected by NCBI online analysis tool BLSATN. MEGA-X was used to build a wgSNP phylogenetic tree.

Results:

GD-Guangzhou-2010 was Belfanti and GD-Zuhai-2020 was Gravis. ANIb between GD-Guangzhou-2010 and C. belfantii was 99.61%. ANI between GD-Zhuhai-2020 and C. diphtheriae was 97.64%. BLASTN results showed that the nitrate reduction gene narKGHIJ and tox gene of GD-Guangzhou-2010 was negative, while GD-Zhuhai-2020 nitrate reduction gene narKGHIJ was positive. There were two obvious clades in wgSNP phylogenetic tree. The first clades included all Mitis and Gravis types strains as well as GD-Zhuhai-2020. The second clades contained all isolates of C.belfantii, C.diphtheriae subsp. lausannense and GD-guangzhou-2010.

Conclusion:

Two non-toxic C. diphtheriae strains are successfully isolated and identified. The phylogenetic tree suggests that GD-Guangzhou-2010 and GD-Zhuhai-2020 are located in two different evolutionary branches.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / China / Corynebacterium / Corynebacterium diphtheriae / Diphtheria / Nitrates Type of study: Diagnostic study Limits: Humans Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Preventive Medicine Year: 2022 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / China / Corynebacterium / Corynebacterium diphtheriae / Diphtheria / Nitrates Type of study: Diagnostic study Limits: Humans Country/Region as subject: Asia Language: Chinese Journal: Chinese Journal of Preventive Medicine Year: 2022 Type: Article