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Anti-tumor Effect and Mechanism of Lycopus lucidus Polysaccharide on Non-small Cell Lung Cancer A549 Cells / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae ; (24): 83-90, 2022.
Article in Chinese | WPRIM | ID: wpr-940555
ABSTRACT
ObjectiveTo study the anti-tumor activity and mechanism of Lycopus lucidus polysaccharide (LLP) in vitro. MethodCell counting kit-8 (CCK-8) assay was used to detect the inhibitory effect of LLP (0, 5, 10, 15, 20 g·L-1) on the proliferation of A549 cells at different time points (24,48,72 h). The migration and invasion abilities of A549 cells were detected by wound healing assay and transwell assay after LLP (10, 20 g·L-1) treatment for 24,48 h. Propidium iodide (PI) single staining was applied to determine the effect of LLP of different concentrations (10,20 g·L-1) on the cell cycle of A549. The apoptosis of A549 cells induced by LLP (10, 20 g·L-1) was detected by Annexin V-FITC/PI kit. Real-time fluorescence-based quantitative polymerase chain reactionReal-time PCR) was adopted to measure effect of LLP (10, 20 g·L-1) on gene expression of cysteine aspartate protease-3 (Caspase-3),cysteine aspartate protease-8 (Caspase-8),cysteine aspartate protease-9 (Caspase-9),cyclin-dependent kinase-1 (CDK-1), and Cyclin B1 in A549 cells. Western blot was used to detect the effect of LLP on protein expression of Caspase-3Caspase-8Caspase-9B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax),CDK-1,cyclin-dependent kinase-4 (CDK-4),cyclin-dependent kinase-6 (CDK-6),Cyclin B1,and Cyclin D1 in A549 cells. ResultCompared with the blank group, the LLP group showed decreased proliferation, migration, and invasion of A549 cells (P<0.05, P<0.01), increased proportion of G0/G1 phase (P<0.05), enhanced apoptosis rate (P<0.05, P<0.01), elevated mRNA expression of Caspase-3Caspase-8,and Caspase-9 (P<0.05,P<0.01), reduced mRNA expression of CDK-1 and Cyclin B1 (P<0.05,P<0.01), up-regulated protein expression of Caspase-3Caspase-8Caspase-9, and Bax (P<0.05, P<0.01), and down-regulated protein expression of Bcl-2, CDK-1, CDK-4, CDK-6, Cyclin B1, and Cyclin D1 (P<0.05, P<0.01). ConclusionLLP can inhibit the proliferation of A549 cells, block the cell cycle in the G0/G1 phase (also G2/M phase), and induce cell apoptosis via the mitochondrial apoptosis pathway and death receptor pathway.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2022 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2022 Type: Article