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Therapeutic Effect of Buyang Huanwutang on Diabetic Peripheral Neuropathy Rats from Perspective of Oxidative Stress / 中国实验方剂学杂志
Article in Zh | WPRIM | ID: wpr-940654
Responsible library: WPRO
ABSTRACT
ObjectiveTo explore the neuroprotective mechanism of Buyang Huanwutang (BYHW) on diabetic peripheral neuropathy (DPN) rats based on oxidative stress and investigate the dosage of Astragali Radix (AR). MethodNinety SD rats were randomly divided into a normal group, a model group, an α-lipoic acid group (60 mg·kg-1·d-1), and BYHW groups with high- (15 g·kg-1·d-1), medium- (8.75 g·kg-1·d-1), and low-dose (5.625 g·kg-1·d-1) AR groups. The diabetes model was induced in rats except for those in the normal group by the high-sugar/high-fat diet and intraperitoneal injection of streptozotocin (STZ). Drug intervention lasted for 12 weeks. The paw withdrawal threshold (PWT) and sensory nerve conduction velocity (SNCV) were detected after drug intervention. Gonad-stimulating hormone (GSH) and malondialdehyde (MDA) were determined. The mitochondrial morphology and structure in sensory neurons of L4-5 dorsal root ganglion (DRG) of rats were observed by electron microscopy. Respiratory chain complex Ⅰ, Ⅱ, Ⅲ, and Ⅳ activities and the mitochondrial membrane potential were detected. The main proteins in the adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor-related factor-2 (Nrf2) pathway, such as phosphorylated AMPK (p-AMPK), phosphorylated Nrf2(p-Nrf2), heme oxygenase-1 (HO-1), and quinone NADH dehydrogenase 1 (NQO1), were detected by immunohistochemistry and Western blot. ResultCompared with the normal group, the model group showed increased fasting blood glucose (P<0.01), decreased content of SNCV, PWT, and GSH (P<0.01), elevated MDA content (P<0.01), obvious mitochondrial damage with vacuolations, reduced activities of respiratory chain complex Ⅰ, Ⅱ, Ⅲ, and Ⅳ and mitochondrial membrane potential (P<0.01), and declining p-AMPK, p-Nrf2, HO-1, and NQO1 (P<0.01). Compared with the model group, the α-lipoic acid group and BYHW high-dose group showed increased SNCV, PWT, and GSH, decreased MDA (P<0.05, P<0.01), alleviated mitochondrial structural damage, increased respiratory chain complex Ⅰ, Ⅱ, Ⅲ, and Ⅳ activities and mitochondrial membrane potential (P<0.01), and elevated p-AMPK, p-Nrf2, HO-1, and NQO1 (P<0.05, P<0.01). ConclusionBYHW regulates oxidative stress through the AMPK/Nrf2 pathway to treat DPN. The therapeutic effect of BYHW is related to the dosage of AR. The BYHW group with high-dose AR is superior to the BYHW groups with medium- and low-dose AR groups in inhibiting oxidative stress.
Key words
Full text: 1 Index: WPRIM Type of study: Prognostic_studies Language: Zh Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2022 Type: Article
Full text: 1 Index: WPRIM Type of study: Prognostic_studies Language: Zh Journal: Chinese Journal of Experimental Traditional Medical Formulae Year: 2022 Type: Article