Determination of ethyl glucuronide in blood and urine by LC-MS/MS / 法医学杂志

ABSTRACT

OBJECTIVE@#To develop a method for determining ethyl glucuronide (EtG) in blood and urine by liquid chromatograph coupled with tandem mass spectrometer (LC-MS/MS).@*METHODS@#After blood and urine de-proteined by acetonitrile, the supernate obtained from a centrifuge was analyzed by LC-MS/MS.@*RESULTS@#Determination limit of EtG in both blood and urine was 0.05 pg/mL, with a linear range of 0.10-5.00 microg/mL (r > 0.999). Accuracy in both matrixes was 95%-109%. Inter- and intra-day RSD were less than 12%. The method showed an excellent performance when it was used to analyze authentic blood and urine samples for EtG.@*CONCLUSION@#The method is capable for blood and urine EtG analysis.