Simultaneous determination of sixteen antibiotics in human urine with ultra performance liquid chromatography-tandem mass spectrometry / 法医学杂志
Journal of Forensic Medicine
; (6): 25-29, 2011.
Article
in Zh
| WPRIM
| ID: wpr-983620
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To develop a method for simultaneous determination of sixteen antibiotics in human urine by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).@*METHODS@#With Piperacillin as an internal standard, the target antibiotics in urine samples were enriched and purified by Oasis HLB solid phase extraction (SPE) cartridges, then separated in a ZORBAX SB-C18 column with a gradient elution of mobile phase of 0.1% formic acid water and acetonitrile, finally analyzed with multiple reaction monitoring (MRM) mode.@*RESULTS@#The limits of detection (LOD) for these sixteen antibiotics were in the range of 0.05-10.0 ng/mL and the limits of quantification (LOQ) in the range of 0.25-20.0 ng/mL. Within the related linear range, the related coefficient (r) of sixteen antibiotics were all more than 0.995. Accuracies for these antibiotics were ranged from 82.0%-119.3%, the within-day precision were less than 13.9%.@*CONCLUSION@#The developed method is sensitive, specific and appropriate for the analysis of antibiotics in forensic toxicology and therapeutic drugs monitoring.
Full text:
1
Index:
WPRIM
Main subject:
Penicillins
/
Solvents
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Time Factors
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Reproducibility of Results
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Chromatography, High Pressure Liquid
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Sensitivity and Specificity
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Spectrometry, Mass, Electrospray Ionization
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Tandem Mass Spectrometry
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Solid Phase Extraction
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Forensic Toxicology
Limits:
Humans
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2011
Type:
Article