Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis / 法医学杂志
Journal of Forensic Medicine
; (6): 41-43, 2012.
Article
in Zh
| WPRIM
| ID: wpr-983711
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity.@*METHODS@#DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system.@*RESULTS@#DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30 pg) by pretreatment of DOP-PCR and then detection of STR genotyping.@*CONCLUSION@#The system established in this study is reliable and more testing sensitive for forensic trace evidence.
Full text:
1
Index:
WPRIM
Main subject:
Oligonucleotides
/
DNA
/
Gene Amplification
/
Polymerase Chain Reaction
/
Reproducibility of Results
/
DNA Fingerprinting
/
Sensitivity and Specificity
/
DNA Primers
/
Electrophoresis, Capillary
/
Tandem Repeat Sequences
Limits:
Humans
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2012
Type:
Article