Altered expression of gamma-secretase components in animal model of major depressive disorder induced by reserpine administration / 한국실험동물학회지
Laboratory Animal Research
; : 109-114, 2012.
Article
in En
| WPRIM
| ID: wpr-98980
Responsible library:
WPRO
ABSTRACT
Altered expression of neurotrophic factors as well as neuroinflammation is commonly associated with Major depressive disorder (MDD) and Alzheimer's disease (AD). To investigate whether or not reserpine-induced MDD affects the expression of AD-related proteins, the expression of gamma-secretase components and substrate were measured in brains of ICR mice following reserpine treatment for 15 days. In active avoidance test, total response time and peak slightly increased in the 2 mg/kg reserpine (RSP2)-treated group compared to vehicle-treated group (P<0.05). Expression and phosphorylation of MKP-1, which is a key factor in MDD pathology, were both higher in the RSP2-treated group than the vehicle- and 1 mg/kg reserpine (RSP1)-treated groups (P<0.02). Furthermore, full-length expression of amyloid precursor protein (APP) was enhanced in the RSP1 and RSP2-treated groups compared to the vehicle-treated group, whereas expression of gamma-secretase components decreased (P<0.03). Among the three components of the gamma-secretase complex, nicastrin protein underwent the largest decrease in expression, as detected by Western blotting (P<0.03). Therefore, the data presented here provide additional evidence about the pathological correlation between MDD and AD.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phosphorylation
/
Reaction Time
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Reserpine
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Brain
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Membrane Glycoproteins
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Proteins
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Blotting, Western
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Models, Animal
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Depressive Disorder, Major
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Amyloid Precursor Protein Secretases
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Laboratory Animal Research
Year:
2012
Type:
Article