Rapid detection of sepsis using cesda: the caenorhabditis elegans sepsis detection assay
Rev. Soc. Bras. Med. Trop
; 52: e20180300, 2019. tab, graf
Article
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| LILACS
| ID: biblio-1041561
Biblioteca responsable:
BR1.1
ABSTRACT
Abstract INTRODUCTION The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. RESULTS The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). CONCLUSIONS CESDA could be further explored for sepsis diagnosis.
Palabras clave
Texto completo:
1
Índice:
LILACS
Asunto principal:
Biomarcadores
/
Quimiotaxis
/
Caenorhabditis elegans
/
Sepsis
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
MEDICINA TROPICAL
Año:
2019
Tipo del documento:
Article