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Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients
Ferreira, Thiago de Oliveira; Koto, Rafael Yoshio; Leite, Gabriel Fialkovitz da Costa; Klautau, Giselle Burlamaqui; Nigro, Stanley; Silva, Cely Barreto da; Souza, Ana Paula Idalgo da Fonseca; Mimica, Marcelo Jenne; Cesar, Regina Grigolli; Salles, Mauro José Costa.
Afiliación
  • Ferreira, Thiago de Oliveira; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
  • Koto, Rafael Yoshio; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
  • Leite, Gabriel Fialkovitz da Costa; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
  • Klautau, Giselle Burlamaqui; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
  • Nigro, Stanley; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
  • Silva, Cely Barreto da; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
  • Souza, Ana Paula Idalgo da Fonseca; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
  • Mimica, Marcelo Jenne; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
  • Cesar, Regina Grigolli; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
  • Salles, Mauro José Costa; Santa Casa de São Paulo. Escola de Ciências Médicas. Departamento de Medicina Interna. São Paulo. BR
Braz. j. infect. dis ; Braz. j. infect. dis;20(5): 468-475, Sept.-Oct. 2016. tab, graf
Article en En | LILACS | ID: biblio-828141
Biblioteca responsable: BR1.1
ABSTRACT
Abstract Objectives To compare cultured microorganisms identified on endotracheal tubes biofilms through sonication technique with traditional tracheal aspirate collected at extubation of pediatric intensive care unit patients. Methods Demographic and epidemiological data were analyzed to identify factors possibly related with the microbiological profile of the two collection methods. Associations between categorical and continuous variables were analyzed using the chi-square or Fisher's exact test, or Student's t test. p-Value <0.05 were considered significant. Results Thirty endotracheal tubes and tracheal aspirates samples from 27 subjects were analyzed. Only one patient presented the clinical diagnosis of ventilator-associated pneumonia. Overall, 50% of bacteria were Gram-negative bacilli, followed by Gram-positive bacteria in 37%, and fungi in 10%. No statistically significant difference on the distribution of Gram-positive or Gram-negative bacteria (p = 0.996), and fungi (p = 0.985) were observed between the collection methods. Pseudomonas spp. was the most frequent microorganism identified (23.8%), followed by Streptococcus spp. (18.5%), Acinetobacter spp. (15.9%), coagulase-negative staphylococci (11.2%), and Klebsiella spp. (8.6%). Concordant results between methods amounted to 83.3%. Pseudomonas aeruginosa and Acinetobacter baumannii showed carbapenem resistance in 50% and 43.7% of the isolates, respectively. In general, cultures after endotracheal tubes sonication (non-centrifuged sonication fluid and centrifuged sonication fluid) yielded bacteria with higher rates of antimicrobial resistance compared to tracheal aspirates cultures. Additionally, in 12 subjects (40%), we observed discrepancies regarding microbiologic profiles of cultures performed using the collection methods. Conclusions Our study demonstrated that sonication technique can be applied to ET biofilms to identify microorganisms attached to their surface with a great variety of species identified. However, we did not find significant differences in comparison with the traditional tracheal aspirate culture approach.
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Texto completo: 1 Índice: LILACS Asunto principal: Sonicación / Unidades de Cuidado Intensivo Pediátrico / Biopelículas / Equipos y Suministros de Hospitales / Intubación Intratraqueal Tipo de estudio: Prognostic_studies Límite: Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: Braz. j. infect. dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2016 Tipo del documento: Article

Texto completo: 1 Índice: LILACS Asunto principal: Sonicación / Unidades de Cuidado Intensivo Pediátrico / Biopelículas / Equipos y Suministros de Hospitales / Intubación Intratraqueal Tipo de estudio: Prognostic_studies Límite: Child / Child, preschool / Female / Humans / Infant / Male Idioma: En Revista: Braz. j. infect. dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2016 Tipo del documento: Article