Rapid, reliable and inexpensive quality assessment of biotinylated cRNA
Braz. j. med. biol. res
; 39(5): 589-593, May 2006. ilus, tab
Article
en En
| LILACS
| ID: lil-425790
Biblioteca responsable:
BR1.1
RESUMO
The interpretation of oligonucleotide array experiments depends on the quality of the target cRNA used. cRNA target quality is assessed by quantitative analysis of the representation of 5' and 3' sequences of control genes using commercially available Test arrays. The Test array provides an economically priced means of determining the quality of labeled target prior to analysis on whole genome expression arrays. This manuscript validates the use of a duplex RT-PCR assay as a faster (6 h) and less expensive (
Texto completo:
1
Índice:
LILACS
Asunto principal:
ARN Complementario
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Análisis de Secuencia por Matrices de Oligonucleótidos
Límite:
Humans
Idioma:
En
Revista:
Braz. j. med. biol. res
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
2006
Tipo del documento:
Article
/
Project document