Cloning of high molecular weight gluten subunit promoter and study on its function in wheat
Braz. arch. biol. technol
; 52(2): 265-270, Mar.-Apr. 2009. ilus, graf
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| LILACS
| ID: lil-513245
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ABSTRACT
The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.
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LILACS
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Braz. arch. biol. technol
Asunto de la revista:
BIOLOGIA
Año:
2009
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Article