Correlation between serum HDAC3 and SMAD3 levels and the degree of neurological deficit in patients with acute ischemic stroke / 中风与神经疾病杂志

ABSTRACT

@#Objective To explore the correlation between serum levels of histone deacetylase 3(HDAC3) and the signal molecule SMAD family member 3(SMAD3) and the degree of neurological deficit in patients with acute ischemic stroke(AIS). Methods From June 2021 to June 2022,104 patients diagnosed with AIS in our hospital were selected. Using the National Institutes of Health Stroke Scale(NIHSS),they were divided based on the degree of neurological impairment into mild group(32 cases),moderate group(37 cases),and severe group(35 cases). At the same time,98 healthy volunteers from health examination in our hospital were included as the control group. The expression levels of HDAC3,SMAD3,tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6) in serum were measured by using enzyme-linked immunosorbent assay. Pearson correlation analysis was used to analyze the correlation between the expression of HDAC3 and SMAD3.Spearman correlation analysis was used to analyze the correlation between the expression of HDAC3 and SMAD3 and NIHSS score and the degree of neurological deficit. Results Compared with the control group,the AIS group showed significantly higher levels of triglyceride,total cholesterol,low-density lipoprotein cholesterol,fibrinogen,TNF-α,IL-1β,and IL-6 and a significantly lower level of HDL-C(all P<0.05). HDAC3 and SMAD3 levels were significantly higher in the AIS group than in the control group(both P<0.05). HDAC3 and SMAD3 levels in the severe group were significantly higher than those in the moderate and mild groups,and those in the moderate group were significantly higher than those in the mild group(all P<0.05). The Pearson correlation analysis showed that the serum expression levels of HDAC3 and SMAD3 in the AIS patients were positively correlated(P<0.05). The Spearman correlation analysis showed positive correlations between the expression of HDAC3 and SMAD3 and NIHSS score(r=0.339,0.563;both P<0.001)and the degree of neurological impairment(r=0.698,0.572;both P<0.001). Conclusion Serum HDAC3 and SMAD3 levels in patients with AIS were abnormally increased,and both were related to the degree of neurological deficit.