The Beneficial Effect of Korean Red Ginseng on Rat Bladder Contractility and Oxidant Damage Following Ischemia/Reperfusion
Journal of the Korean Continence Society
; : 142-151, 2009.
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| ID: wpr-106840
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ABSTRACT
PURPOSE:
N-acetylcysteine (NAC) is a potent antioxidant, and a free radical scavenger. We investigated the possible effects of NAC after ischemia/reperfusion (I/R) of rat bladder. MATERIALS ANDMETHODS:
I/R injury was induced by abdominal aorta clamping and ischemia for 60minutes, followed by 120minutes reperfusion. Twenty rats were divided into four groups sham operation + saline group (S+S), sham operation + NAC group (S+NAC), I/R + saline group (I/R+S), I/R + NAC group (I/R+NAC). Blood levels of reactive oxygen species (ROS) were determined using the free oxygen radical tests (FORT). Superoxide generation was measured based on lucigenin-enhanced chemiluminescence. The level of malondialdehyde (MDA) was analyzed in order to measure lipid peroxidation.RESULTS:
In I/R+S group, the isometric contractile responses to carbachol were significant lower than other groups and were reversed by the pretreatment with NAC. The level of FORT and MDA showed a marked increase in I/R+S group compared with S+S group. NADPH-stimulated superoxide production was also significantly increased. I/R+NAC decreased these parameters compared with I/R+S group.CONCLUSION:
Our results suggest that treatment with NAC reversed the low contractile responses of rat bladder and prevented oxidative stress following I/R.Palabras clave
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Asunto principal:
Aorta Abdominal
/
Oxígeno
/
Acetilcisteína
/
Vejiga Urinaria
/
Carbacol
/
Peroxidación de Lípido
/
Reperfusión
/
Especies Reactivas de Oxígeno
/
Superóxidos
/
Estrés Oxidativo
Límite:
Animals
Idioma:
En
Revista:
Journal of the Korean Continence Society
Año:
2009
Tipo del documento:
Article