Ethacrynic Acid Inhibits Sphingosylphosphorylcholine-Induced Keratin 8 Phosphorylation and Reorganization via Transglutaminase-2 Inhibition
Biomolecules & Therapeutics
; : 338-342, 2013.
Article
en En
| WPRIM
| ID: wpr-108280
Biblioteca responsable:
WPRO
ABSTRACT
Sphingosylphosphorylcholine (SPC) is significantly increased in the malicious ascites of tumor patients and induces perinuclear reorganization of keratin 8 (K8) filaments in PANC-1 cells. The reorganization contributes to the viscoelasticity of metastatic cancer cells resulting in increased migration. Recently, we reported that transglutaminase-2 (Tgase-2) is involved in SPC-induced K8 phosphorylation and reorganization. However, effects of Tgase-2 inhibitors on SPC-induced K8 phosphorylation and reorganization were not clearly studied. We found that ethacrynic acid (ECA) concentration-dependently inhibited Tgase-2. Therefore, we examined the effects of ECA on SPC-induced K8 phosphorylation and reorganization. ECA concentration-dependently suppressed the SPC-induced phosphorylation and perinuclear reorganization of K8. ECA also suppressed the SPC-induced migration and invasion. SPC induced JNK activation through Tgase-2 expression and ECA suppressed the activation and expression of JNK in PANC-1 cells. These results suggested that ECA might be useful to control Tgase-2 dependent metastasis of cancer cells such as pancreatic cancer and lung cancers.
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Texto completo:
1
Índice:
WPRIM
Asunto principal:
Neoplasias Pancreáticas
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Fosforilación
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Ascitis
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Ácido Etacrínico
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Queratina-8
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Neoplasias Pulmonares
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Metástasis de la Neoplasia
Límite:
Humans
Idioma:
En
Revista:
Biomolecules & Therapeutics
Año:
2013
Tipo del documento:
Article