Effects of Histone Deacetylase Inhibitor (Valproic Acid) on the Expression of Hypoxia-inducible Factor-1 Alpha in Human Retinal Müller Cells
Korean Journal of Ophthalmology
; : 80-85, 2017.
Article
en En
| WPRIM
| ID: wpr-122711
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WPRO
ABSTRACT
PURPOSE:
To evaluate the effects of valproic acid (VPA), a histone deacetylase inhibitor (HDACI), on the expression of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in human retinal Müller cells under hypoxic conditions.METHODS:
Chemical hypoxia was induced in human retinal Müller cells (MIO-M1) by treatment with increasing concentrations of cobalt(II) chloride (CoCl₂). Müller cells were also treated with a set concentration of CoCl₂, along with various concentrations of VPA. The expression of HIF-1α and VEGF in the treated Müller cells was determined by enzyme-linked immunosorbent assay.RESULTS:
Exposure of human retinal Müller cells to increasing concentrations of CoCl₂ produced a dose-dependent increase in HIF-1α expression. The addition of increasing concentrations of VPA lead to a dose-dependent decrease in expression of HIF-1α and VEGF in Müller cells exposed to a set concentration of CoCl₂.CONCLUSIONS:
HDACI VPA downregulated the expressions of HIF-1α and VEGF in human retinal Müller cells under hypoxic conditions. Using HDACI to target HIF-1α expression in Müller cells could be a new therapeutic strategy for the treatment of retinal vascular diseases.Palabras clave
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Asunto principal:
Retinaldehído
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Enfermedades Vasculares
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Ensayo de Inmunoadsorción Enzimática
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Histonas
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Ácido Valproico
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Factor A de Crecimiento Endotelial Vascular
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Inhibidores de Histona Desacetilasas
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Células Ependimogliales
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Histona Desacetilasas
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Hipoxia
Límite:
Humans
Idioma:
En
Revista:
Korean Journal of Ophthalmology
Año:
2017
Tipo del documento:
Article