Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China
The Korean Journal of Parasitology
; : 119-124, 2015.
Article
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| WPRIM
| ID: wpr-130541
Biblioteca responsable:
WPRO
ABSTRACT
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), beta-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Filogenia
/
ADN Ribosómico
/
Datos de Secuencia Molecular
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ARN Ribosómico 18S
/
Enfermedades de los Gatos
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Análisis por Conglomerados
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Proteínas Protozoarias
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China
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ADN Protozoario
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Giardiasis
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
The Korean Journal of Parasitology
Año:
2015
Tipo del documento:
Article