N-ras Mutation Detection by Pyrosequencing in Adult Patients with Acute Myeloid Leukemia at a Single Institution
Annals of Laboratory Medicine
; : 159-166, 2013.
Article
en En
| WPRIM
| ID: wpr-144114
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND: N-ras mutations are one of the most commonly detected abnormalities of myeloid origin. N-ras mutations result in a constitutively active N-ras protein that induces uncontrolled cell proliferation and inhibits apoptosis. We analyzed N-ras mutations in adult patients with AML at a particular institution and compared pyrosequencing analysis with a direct sequencing method for the detection of N-ras mutations. METHODS: We analyzed 90 bone marrow samples from 83 AML patients. We detected N-ras mutations in codons 12, 13, and 61 using the pyrosequencing method and subsequently confirmed all data by direct sequencing. Using these methods, we screened the N-ras mutation quantitatively and determined the incidence and characteristic of N-ras mutation. RESULTS: The incidence of N-ras mutation was 7.2% in adult AML patients. The patients with N-ras mutations showed significant higher hemoglobin levels (P=0.022) and an increased incidence of FLT3 mutations (P=0.003). We observed 3 cases with N-ras mutations in codon 12 (3.6%), 2 cases in codon 13 (2.4%), and 1 case in codon 61 (1.2%). All the mutations disappeared during chemotherapy. CONCLUSIONS: There is a low incidence (7.2%) of N-ras mutations in AML patients compared with other populations. Similar data is obtained by both pyrosequencing and direct sequencing. This study showed the correlation between the N-ras mutation and the therapeutic response. However, pyrosequencing provides quantitative data and is useful for monitoring therapeutic responses.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Médula Ósea
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Codón
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Hemoglobinas
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Leucemia Mieloide Aguda
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Incidencia
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Análisis de Secuencia de ADN
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Proteínas ras
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Análisis Citogenético
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Tirosina Quinasa 3 Similar a fms
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Mutación
Tipo de estudio:
Diagnostic_studies
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Incidence_studies
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Prognostic_studies
Límite:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Idioma:
En
Revista:
Annals of Laboratory Medicine
Año:
2013
Tipo del documento:
Article