Molecular identification of Mycoplasma cynos from laboratory beagle dogs with respiratory disease / 한국실험동물학회지
Laboratory Animal Research
; : 61-66, 2012.
Article
en En
| WPRIM
| ID: wpr-145351
Biblioteca responsable:
WPRO
ABSTRACT
In this study, we examined a colony of 20 beagle dogs in a laboratory animal facility. Mycoplasma was detected by consensus PCR assay in 1 dog with respiratory and constitutional symptoms. None of the other dogs were affected. The dog was euthanized and necropsied. In postmortem examinations, gray or plum-colored gross lesions were found on the lung, most commonly in the apical and cardiac lobes. Some lesions showed clear demarcation and consolidation. Microscopic examination showed peribronchiolar lymphoid hyperplasia and interstitial thickening, lesions pathognomonic for mycoplasma pneumonia. To identify canine Mycoplasma species, we used species-specific PCR reactions for M. arginini, M. canis, M. cynos, M. edwardii, M. felis, M. gateae, M. maculosum, M. molare, M. opalescens, M. spumans, Mycoplasma sp. HRC 689, and M. collis. As the result, we identified Mycoplasma cynos by amplification of DNA extracted from lung tissue of the laboratory beagle dog with respiratory disease.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Neumonía por Mycoplasma
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Autopsia
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ADN
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Reacción en Cadena de la Polimerasa
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Consenso
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Felis
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Hiperplasia
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Pulmón
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Animales de Laboratorio
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Diente Molar
Tipo de estudio:
Diagnostic_studies
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Guideline
Límite:
Animals
Idioma:
En
Revista:
Laboratory Animal Research
Año:
2012
Tipo del documento:
Article