Imaging of Herpes Simplex Virus Type 1 Thymidine Kinase Gene Expression with Radiolabeled 5-(2-iodovinyl)-2'-deoxyuridine (IVDU) in Liver by Hydrodynamic-based Procedure / 핵의학분자영상
Nuclear Medicine and Molecular Imaging
; : 468-477, 2009.
Article
en Ko
| WPRIM
| ID: wpr-155611
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE:
Hydrodynamic-based procedure is a simple and effective gene delivery method to lead a high gene expression in liver tissue. Non-invasive imaging reporter gene system has been used widely with herpes simplex virus type 1 thymidine kinase (HSV1-tk) and its various substrates. In the present study, we investigated to image the expression of HSV1-tk gene with 5-(2-iodovinyl)-2'-deoxyuridine (IVDU) in mouse liver by the hydrodynamicbased procedure. MATERIALS ANDMETHODS:
HSV1-tk or enhanced green fluorescence protein (EGFP) encoded plasmid DNA was transferred into the mouse liver by hydrodynaminc injection. At 24 h post-injection, RT-PCR, biodistribution, fluorescence imaging, nuclear imaging and digital wholebody autoradiography (DWBA) were performed to confirm transferred gene expression.RESULTS:
In RT-PCR assay using mRNA from the mouse liver, specific bands of HSV1-tk and EGFP gene were observed in HSV1-tk and EGFP expressing plasmid injected mouse, respectively. Higher uptake of radiolabeled IVDU was exhibited in liver of HSV1-tk gene transferred mouse by biodistribution study. In fluorescence imaging, the liver showed specific fluorescence signal in EGFP gene transferred mouse. Gamma-camera image and DWBA results showed that radiolabeled IVDU was accumulated in the liver of HSV1-tk gene transferred mouse.CONCLUSION:
In this study, hydrodynamic-based procedure was effective in liver-specific gene delivery and it could be quantified with molecular imaging methods. Therefore, co-expression of HSV1-tk reporter gene and target gene by hydrodynamic-based procedure is expected to be a useful method for the evaluation of the target gene expression level with radiolabeled IVDU.Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Plásmidos
/
Poliestirenos
/
Autorradiografía
/
Timidina Quinasa
/
ADN
/
ARN Mensajero
/
Expresión Génica
/
Simplexvirus
/
Herpesvirus Humano 1
/
Genes Reporteros
Límite:
Animals
Idioma:
Ko
Revista:
Nuclear Medicine and Molecular Imaging
Año:
2009
Tipo del documento:
Article