Equine hyperimmune serum protects mice against Clostridium difficile spore challenge
Journal of Veterinary Science
; : 249-258, 2014.
Article
en En
| WPRIM
| ID: wpr-191843
Biblioteca responsable:
WPRO
ABSTRACT
Clostridium (C.) difficile is a common cause of nosocomial diarrhea in horses. Vancomycin and metronidazole have been used as standard treatments but are only moderately effective, which highlights the need for a novel alternative therapy. In the current study, we prepared antiserum of equine origin against both C. difficile toxins A and B as well as whole-cell bacteria. The toxin-neutralizing activities of the antibodies were evaluated in vitro and the prophylactic effects of in vivo passive immunotherapy were demonstrated using a conventional mouse model. The data demonstrated that immunized horses generated antibodies against both toxins A and B that possessed toxin-neutralizing activity. Additionally, mice treated with the antiserum lost less weight without any sign of illness and regained weight back to a normal range more rapidly compared to the control group when challenged orally with 10(7) C. difficile spores 1 day after serum injection. These results indicate that intravenous delivery of hyperimmune serum can protect animals from C. difficile challenge in a dose-dependent manner. Hence, immunotherapy may be a promising prophylactic strategy for preventing C. difficile infection in horses.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Esporas Bacterianas
/
Proteínas Bacterianas
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Toxinas Bacterianas
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Clostridioides difficile
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Inmunización Pasiva
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Infecciones por Clostridium
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Enterotoxinas
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Enfermedades de los Caballos
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Caballos
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Sueros Inmunes
Límite:
Animals
Idioma:
En
Revista:
Journal of Veterinary Science
Año:
2014
Tipo del documento:
Article