GFP-tagged E. coli shows bacterial distribution in mouse organs: pathogen tracking using fluorescence signal
Clinical and Experimental Vaccine Research
; : 83-87, 2012.
Article
en En
| WPRIM
| ID: wpr-192288
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: In vaccine efficacy evaluation, visualization of pathogens in whole organism at each time point would be able to reduce the consuming animals and provide the in vivo information within consistent background with identical organism. MATERIALS AND METHODS: Using IVIS spectrum whole live-animal imaging system, fluorescent intensity was optimized and visualized proportionately by concentrating Escherichia coli MC1061 strain which expresses GFP (E. coli-GFP) in BALB/C mice after injection. RESULTS: Local distribution of disseminated E. coli-GFP was traced in each organ by fluorescence. Detached organ showed more obvious fluorescent signal, and intestine showed strongest fluorescent signal. CONCLUSION: This in vivo imaging method using GFP-tagged pathogen strain suggest quantified infected pathogens by fluorescence intensity in whole animals can provide the information about the localization and distribution after infection.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Esguinces y Distensiones
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Infecciones Bacterianas
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Atletismo
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Escherichia coli
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Imagen Molecular
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Fluorescencia
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Intestinos
Límite:
Animals
Idioma:
En
Revista:
Clinical and Experimental Vaccine Research
Año:
2012
Tipo del documento:
Article