Compiling Multicopy Single-Stranded DNA Sequences from Bacterial Genome Sequences
Genomics & Informatics
; : 29-33, 2016.
Article
en En
| WPRIM
| ID: wpr-193407
Biblioteca responsable:
WPRO
ABSTRACT
A retron is a bacterial retroelement that encodes an RNA gene and a reverse transcriptase (RT). The former, once transcribed, works as a template primer for reverse transcription by the latter. The resulting DNA is covalently linked to the upstream part of the RNA; this chimera is called multicopy single-stranded DNA (msDNA), which is extrachromosomal DNA found in many bacterial species. Based on the conserved features in the eight known msDNA sequences, we developed a detection method and applied it to scan National Center for Biotechnology Information (NCBI) RefSeq bacterial genome sequences. Among 16,844 bacterial sequences possessing a retron-type RT domain, we identified 48 unique types of msDNA. Currently, the biological role of msDNA is not well understood. Our work will be a useful tool in studying the distribution, evolution, and physiological role of msDNA.
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1
Índice:
WPRIM
Asunto principal:
Biotecnología
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ADN
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ADN de Cadena Simple
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ARN
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Quimera
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ADN Polimerasa Dirigida por ARN
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Genoma Bacteriano
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Retroelementos
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Transcripción Reversa
Idioma:
En
Revista:
Genomics & Informatics
Año:
2016
Tipo del documento:
Article