PDCD5 induces the apoptosis of human prostate cancer cells PC-3M-1E8 / 中华男科学杂志
National Journal of Andrology
; (12): 979-982, 2007.
Article
en Zh
| WPRIM
| ID: wpr-232028
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis-promoting effect of PDCD5 on human prostate cancer cells PC-3M-1E8.</p><p><b>METHODS</b>PCI-neo and PCI-neo-PDCD5 were transfected into PC-3M-1E8 cells by Lipofectamine 2000, the viability of the cells was analyzed by MTT assay 16 hours after removal of the serum, and the apoptosis was determined by in situ end-labeling and electron microscopy.</p><p><b>RESULTS</b>The viability and growing speed of the transfected cells were significantly decreased and their apoptotic indexes significantly increased as compared with the control group (P < 0.001).</p><p><b>CONCLUSION</b>PDCD5 may significantly inhibit the in vitro growth and promote the apoptosis of human prostate cancer cells PC-3M-1E8.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Patología
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Fisiología
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Plásmidos
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Neoplasias de la Próstata
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Transfección
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Química
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Apoptosis
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Etiquetado Corte-Fin in Situ
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Línea Celular Tumoral
Límite:
Humans
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Male
Idioma:
Zh
Revista:
National Journal of Andrology
Año:
2007
Tipo del documento:
Article