Small interfering RNA-mediated MAPK p42 silencing induces apoptosis of HeLa cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 11-15, 2006.
Article
en Zh
| WPRIM
| ID: wpr-234206
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of small interfering RNA (siRNA)-induced MAPK p42 silencing on the survival of HeLa cells.</p><p><b>METHODS</b>Two siRNAs targeting at the MAPK p42 gene and one random siRNA were synthesized respectively by Silencer siRNA Construction Kit and transfected into HeLa cells by Lipofectamin 2000. The expression of p42(MAPK) in the transfected HeLa cells was analyzed by Western blotting and immunohistochemistry, and the morphology of cells were observed with electron microscope. TUNEL assay and Annexin V/PI staining were employed for detecting the cell apoptosis.</p><p><b>RESULTS</b>The expression of p42(MAPK) in the HeLa cells was remarkably suppressed after transfection with the two siRNAs, reduced by about 2.5 and 3.2 folds respectively in comparison with the negative control. Chromatin margination in the cell nuclei were observed in the transfected cells, and TUNEL assay and Annexin V/PI staining further confirmed the occurrence of cell apoptosis.</p><p><b>CONCLUSION</b>In vitro MAPK p42 siRNA-1 and siRNA-2 transfection can specifically silence the gene expression and induce apoptosis of HeLa cells.</p>
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Índice:
WPRIM
Asunto principal:
Fisiología
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Células HeLa
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Transfección
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Apoptosis
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Proteína Quinasa 1 Activada por Mitógenos
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Silenciador del Gen
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ARN Interferente Pequeño
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Genética
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Article