A new method to obtain molecular marker of sequence-tagged site (STS) of Panax ginseng and P. quinquefolius / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1012-1015, 2007.
Article
en Zh
| WPRIM
| ID: wpr-235276
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Searching a new molecular method to authenticate Panax ginseng and P. quenquefolium.</p><p><b>METHOD</b>Single primers based on rDNA sequences of Panax species were designed to obtain polymorphic bands of P. ginseng and P. quinquefolius and then sequenced. Four PCR primers (two forword and two reverse primers) specific to P. ginseng and P. quinquefolius were designed.</p><p><b>RESULT</b>Primer Pg-6F, Pg-479R only amplified 474 bp band for P. ginseng and primer Pq-442F, Pq-658R only amplified 217 bp band for P. quinquefolius. It is indicated that the four primers could serve as specific STS primers for Panax species.</p><p><b>CONCLUSION</b>A new way to obtain STS primers of Panax species was established. This method is more quick and efficient than SCAR-PCR method and can serve as a model to obtain molecular markers for other Chinese material medica.</p>
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Asunto principal:
Plantas Medicinales
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Polimorfismo Genético
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Especificidad de la Especie
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ADN Ribosómico
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Datos de Secuencia Molecular
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Secuencia de Bases
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Marcadores Genéticos
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Lugares Marcados de Secuencia
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Clasificación
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Análisis de Secuencia de ADN
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2007
Tipo del documento:
Article