Study on rhG-CSF modified with polyethylene glycol / 生物工程学报
Chinese Journal of Biotechnology
; (12): 965-969, 2005.
Article
en Zh
| WPRIM
| ID: wpr-237042
Biblioteca responsable:
WPRO
ABSTRACT
Monomethoxy Polyethylene Glycol(mPEG20000) was activated by N-hydroxysuccinimede and analyzed by infrared spectrum and hydrolysis kinetics. In order to propose the optimized reaction conditions of mono-PEGylated rhG-CSF, orthogonal design of the experiment was investigated. Ion exchange chromatography was used to separate and purify PEGylated rhG-CSF from unPEGylated rhG-CSF. The purity of mono-PEGylated rhG-CSF was analyzed by high performance liquid chromatography (HPLC) to be 97%.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Polietilenglicoles
/
Propiedades de Superficie
/
Proteínas Recombinantes
/
Química
/
Factor Estimulante de Colonias de Granulocitos
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2005
Tipo del documento:
Article