Experimental study on the effect of sensitized sera on engraftment of hematopoietic stem cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 531-534, 2008.
Article
en Zh
| WPRIM
| ID: wpr-239986
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of sensitized sera on engraftment of hematopoietic stem cells (HSCs) and its mechanism.</p><p><b>METHODS</b>Bone marrow cells (BMCs) from C57BL/6 mice were incubated with sensitized sera (group I) or normal sera (group II), and then washed and transplanted into irradiated BALB/c mice. The survival analysis and engraftment evaluation of the recipients were observed. The incubated BMCs were bound with goat anti mouse IgG for and labeled with Annexin V for apoptosis detection.</p><p><b>RESULTS</b>Seven out of ten recipient mice in group I died of bone marrow failure at day 10 after transplantation, while all of those in group II were long-term survived. Engraftment assay showed recipients blood count and BMCs progressively decreased along with time passing in group I; in addition, the chimeric percentage of donor cells progressively decreased as well. The percentage of BMCs binding with goat anti mouse IgG in group I or group II were (90.3 +/- 5.1)% and (5.2 +/- 2.4)%, respectively, and the difference was statistically significant (P < 0.01). However, no significant difference was found in the apoptosis detection between the two groups (P > 0.05).</p><p><b>CONCLUSION</b>The engraftment capacity of HSCs is significantly impaired by sensitized sera, the antibodies in sensitized sera may bind to antigens expressed on HSCs but do not induce apoptosis.</p>
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Asunto principal:
Patología
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Células de la Médula Ósea
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Células Cultivadas
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Apoptosis
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Anexina A5
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Trasplante de Células Madre Hematopoyéticas
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Sueros Inmunes
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Metabolismo
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Ratones Endogámicos BALB C
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Ratones Endogámicos C57BL
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2008
Tipo del documento:
Article