Effect of adeno-BMP7 transfection on osteogenesis of BMSCs / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of adeno-BMP7 transfection on the biology of bone marrow stromal cells (BMSCs).</p><p><b>METHODS</b>Bone marrow was obtained from the goat. The BMSCs were isolated and cultured at the second passage. Once the cells attached and formed a monolayer with 70%-80% confluency, adeno-BMP7 (M.O.I. = 100) was added to the cells. After three days, calcium node was examined with staining; cell-coral compound was replanted subcutaneously.</p><p><b>RESULTS</b>With adeno-BMP7 transfection, BMP7 expression was detected with Western-blot; big calcium nodes were observed with staining. New bone formation was enhanced, which was evaluated by X-ray and histological examinations.</p><p><b>CONCLUSIONS</b>BMSCs transfected with adeno-BMP7 show much stronger osteogenic ability.</p>