Influence of MicroRNA-382 on Biological Properties of Human Umbilical Cord-Derived Mesenchymal Stem Cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 852-857, 2016.
Article
en Zh
| WPRIM
| ID: wpr-246855
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of microRNA-382 (miR-382) on the biological properties of human umbilical cord-derived mesenchymal stem cells (hUC-MSC).</p><p><b>METHODS</b>The mimics and inhibitor of miR-382 were transfected into hUC-MSC with lipo2000. Inverted microscopy was used to observe the morphology change of hUC-MSC. The proliferation of hUC-MSC was detected by CCK-8. Oil red O and alizarin red staining were applied to assess the adipogenic and osteogenic differentiation of hUC-MSC. Cetylpyridinium chloride was used to the quantitative analysis of osteogenic differentiation. The expression of Runx2 and some cytokines were detected by RT-PCR.</p><p><b>RESULTS</b>miR-382 did not influence the morphology, proliferation and adipogenic differentiation of hUC-MSC miR-382 inhibited the expression of Runx2, thus could inhibit the osteogenesis of hUC-MSC, being confirmed by alizarin red stain; miR-382 could influence the expression of key cytokines secreted from hUC-MSC, such as IL-6, IDO1, G-CSF, M-CSF, GM-CSF.</p><p><b>CONCLUSION</b>miR-382 decreases the expression of Runx2 and inhibites the osteogenesis of hUC-MSC. In addition, it also affects the expression of some key cytokines secreted from hUC-MSC.</p>
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Índice:
WPRIM
Asunto principal:
Osteogénesis
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Cordón Umbilical
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Transfección
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Diferenciación Celular
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Factor Estimulante de Colonias de Granulocitos
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Factor Estimulante de Colonias de Granulocitos y Macrófagos
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Factor Estimulante de Colonias de Macrófagos
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Interleucina-6
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Biología Celular
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MicroARNs
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2016
Tipo del documento:
Article