Synthesis of artificial diethylstilbestrol antigen for preparation of polyclonal antibodies / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 25-31, 2013.
Article
en Zh
| WPRIM
| ID: wpr-252669
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To synthesize artificial diethylstilbestrol (DES) antigen and to prepare DES polyclonal antibody with high titer and sensitivity.</p><p><b>METHODS</b>The derivative of DES (DES-HS) was synthesized from diethylstilbestrol, ethyl bromoacetate,bovine serum albumin (BSA) and chicken ovalbumin (OVA) with the nucleophilic substitution reaction; the compound was identified by electrospray ionization mass spectrometry(ESI-MS). The DES-HS and the carrier proteins (BSA, OVA) were cross-linked to prepare the artificial antigen; the UV absorption spectrophotometry and high performance liquid chromatography (HPLC) were used to identify the prepared artificial antigen. The rabbits were immunized with the DES artificial antigen to prepare the DES polyclonal antibodies.</p><p><b>RESULTS</b>The DES-HS was synthesized. The DES artificial antigen was prepared successfully with a coupling rate of 221. The DES polyclonal antibodies with a titer of 125 600 and IC50 of 10.81 ng/ml were prepared with DES artificial antigen.</p><p><b>CONCLUSION</b>A set of methods to synthesize DES artificial antigen and to prepare the DES polyclonal antibodies has been developed successfully.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
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Química
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Dietilestilbestrol
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Alergia e Inmunología
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Métodos
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Anticuerpos
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Antígenos
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2013
Tipo del documento:
Article