Preparation of salbutamol polyclonal antibodies and development of indirect competitive enzyme-linked immunoassay / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 19-24, 2013.
Article
en Zh
| WPRIM
| ID: wpr-252670
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To prepare the antibodies against salbutamol (SAL) with high sensitivity and to develop an indirect competitive enzyme-linked immunoassay (ic-ELISA) for fast detection of SAL.</p><p><b>METHODS</b>The New Zealand white rabbits were immunized with SAL in a small dose and long period mode. The method of ic-ELISA was optimized and adopted for the detection of a series of SAL samples, then the standard curve of SAL was established. The precision and the recoveries of the method were determined.</p><p><b>RESULTS</b>The antibodies with high sensitivity towards SAL were prepared with a IC50 of 12.21 ng/ml. The ic-ELISA method for SAL measurement was established, the recoveries of measurement was between 95%-105% and the CV was <3%.</p><p><b>CONCLUSION</b>The antibodies against salbutamol have been prepared and an indirect competitive enzyme-linked immunoassay for fast and specific detection of SAL has been developed.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Albuterol
/
Alergia e Inmunología
/
Métodos
/
Anticuerpos
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2013
Tipo del documento:
Article