Study on miR-490-5p and miR-363 as novel biomarkers for the diagnosis of colorectal cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 45-50, 2014.
Article
en Zh
| WPRIM
| ID: wpr-256820
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify dysregulated microRNA(miRNA) that can act as novel biomarker for colorectal cancer screening.</p><p><b>METHODS</b>Real time-polymerase chain reaction was used to detect the expression profile of miRNA in tumor and paired normal tissues, and the significant dysregulated miRNA was examined by non-paired t-test. Receiver operating characteristic(ROC) curve was performed to evaluate the specificity and sensitivity of miR-363 and miR-490-5p as biomarkers to discriminate colorectal cancer patients from normal person.</p><p><b>RESULTS</b>Seven-three dysregulated miRNAs were found in male samples, of which 6 miRNAs were up-regulated and other 67 miRNAs were down-regulated, while 42 dysregulated miRNAs were found in female samples, of which 5 were up-regulated and 37 were down-regulated in tumor tissues compared with normal tissues. Among above dysregulated miRNAs, 33 miRNAs had significant differences, besides, dysregulated expression level of 10 of 33 miRNAs was over 5 folds in male and female as well as mixed samples.</p><p><b>CONCLUSIONS</b>The expression pattern in female is different from that in male. miR-363 and miR-490-5p possess the potential in screening colorectal cancer patients from healthy people.</p>
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Índice:
WPRIM
Asunto principal:
Neoplasias Colorrectales
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Biomarcadores de Tumor
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Estudios de Casos y Controles
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Sensibilidad y Especificidad
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MicroARNs
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Diagnóstico
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Detección Precoz del Cáncer
Tipo de estudio:
Diagnostic_studies
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Observational_studies
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Prognostic_studies
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Screening_studies
Límite:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2014
Tipo del documento:
Article