Analysis of aristolochic acid A from the aerial and underground parts of Asarum by UPLC-UV / 药学学报
Acta Pharmaceutica Sinica
; (12): 221-223, 2008.
Article
en Zh
| WPRIM
| ID: wpr-268142
Biblioteca responsable:
WPRO
ABSTRACT
To develop an UPLC-UV method for the determination of aristolochic acid A in the aerial and underground parts of Asarum, an AcquityUPLC HSST3 column (50 mm x 2.1 mm ID, 1.8 microm) was used with the mobile phase of acetonitrile-0.1% FA and eluted in gradient mode. The flow rate was 0.5 mL min(-1), the detection wavelength was 251 nm, and the column temperature was 40 degrees C. The calibration curve was linear in the range of 18 - 1450 ng of aristolochic acid A with correlation coefficient of 0. 9999 and the minimum detective concentration was 18.25 ng mL (-1). The method developed is accurate and reproducible, and can be applied in the determination of aristolochic acid A in Asarum.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Plantas Medicinales
/
Espectrofotometría Ultravioleta
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Líquida de Alta Presión
/
Sensibilidad y Especificidad
/
Raíces de Plantas
/
Asarum
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Componentes Aéreos de las Plantas
/
Ácidos Aristolóquicos
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2008
Tipo del documento:
Article