Expression of monocarboxylate transporter 8 mRNA in the brain tissue of rats with cerebral ischemia / 南方医科大学学报
Journal of Southern Medical University
; (12): 913-915, 2012.
Article
en Zh
| WPRIM
| ID: wpr-268967
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mRNA expression of monocarboxylate transporter 8 (MCT8), a thyroid hormone transport protein, in the lateral ventricle of rats with cerebral ischemia.</p><p><b>METHODS</b>Immunofluorescence staining was used to observe the expression of MCT8 in the lateral ventricle of 5 normal SD rats. Another 20 adult male SD rats were randomized into 4 groups and subject to permanent ligation of both the common carotid arteries (2-vessel occlusion, 2VO) for 3 days, 2 weeks, or 5 weeks, or no ligation (control). At the end of the experiment, the transcriptional level of MCT8 in the brain tissue of the rats were detected using fluorescent quantitative PCR.</p><p><b>RESULTS</b>MCT8 mRNA levels in 3-day and 2-week 2VO groups were comparable with that in the control group (P=0.909; P=0.694), but increased significantly in 5-week 2VO group compared with that in the control and 3-day 2VO groups (P=0.029; P=0.023). No significance was found in MCT8 mRNA between the 2-week and 5-week 2VO groups (P=0.065).</p><p><b>CONCLUSION</b>Prolonged cerebral ischemia causes compensatory increase of MCT8 mRNA expression on the capillary endothelial cell membranes in the lateral ventricle of rats.</p>
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Índice:
WPRIM
Asunto principal:
Encéfalo
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Infarto Cerebral
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Isquemia Encefálica
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Ratas Sprague-Dawley
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Transportadores de Ácidos Monocarboxílicos
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Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2012
Tipo del documento:
Article