Screening and identification of human anti-c-Met Fab from a phage antibody library / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 505-508, 2008.
Article
en Zh
| WPRIM
| ID: wpr-279757
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen anti-c-Met Fab from a phage antibody library and identify its binding activity.</p><p><b>METHODS</b>The expression of c-Met of HCC lines was identified by Western blot and immunofluorescence. Antibodies against c-Met were screened with immobilized antigen. After five rounds of panning, 30 randomly selected clones were identified by phage ELISA to select specific clones with high affinity. The positive clones were selected for Fab soluble expression in TOP10F and the binding activities were analysed in HCC lines.</p><p><b>RESULTS</b>c-Met expressed in HCC membrane was confirmed by Western blot and immunofluorescence. A Fab fragment named AM2-26 with fine activity to c-Met was selected. AM2-26 binding specificity was confirmed by IP, FACS and immunofluorescence.</p><p><b>CONCLUSION</b>The anti-c-Met Fab binding to c-Met in HCC provides a promising candidate for the biotherapy of hepatoma.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Proteínas Recombinantes de Fusión
/
Región Variable de Inmunoglobulina
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Fragmentos Fab de Inmunoglobulinas
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Biblioteca de Genes
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Clonación Molecular
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Biblioteca de Péptidos
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Proteínas Proto-Oncogénicas c-met
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Línea Celular Tumoral
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Alergia e Inmunología
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Anticuerpos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Año:
2008
Tipo del documento:
Article